We offer microarray-based transcriptomics of one organ or tissue per mouse mutant line. Organs for transcriptome analyses are selected based on the interest of the collaboration partner prior to the arrival of mice at the German Mouse Clinic (GMC). Statistical analyses of the microarray data include the identification of significantly regulated genes and the analysis of their functional annotation. We aid in the biological interpretation of the data by providing enrichment analyses using the Ingenuity Pathway software and GeneRanker software (Genomatix, Germany).
As a standard, organs or tissues that may be selected for transcriptome analyses include brown (scapula) fat, subcutaneous fat, visceral (perigonadal) fat, bone (humerus), whole brain or brain regions (such as cerebellum, cortex, hippocampus, olfactory bulb, spinal cord, or striatum), eye, heart, kidney, liver, lung, (thigh) muscle, pancreas, spleen, salivary gland, auricle (skin and cartilage), ovary, testis, and sperm. 6-8 biological replicates of the organ/tissue per group (typically mutant versus wild-type) are collected at the end of the GMC primary screen, and frozen and stored in liquid nitrogen. Total RNA extraction, amplification, labeling and hybridization to Mouse Clariom Arrays are done according to standardized protocols. Please contact us, if this scheme should be modified for your mouse mutant line.
Dr. Johannes Beckers
Tel. +49 89 3187-3513
Helmholtz Zentrum München -
Deutsches Forschungszentrum für Gesundheit und Umwelt, GmbH
Institute of Experimental Genetics
Ingolstaedter Landstr. 1